Fig 1: Detailed analysis of genes regulated by UTP through P2Y2 receptor activation in cADSC. (A) Enriched biological processes related to UTP target genes after comparison of RNA-sequencing data in WT and P2Y2 KO cADSC. RNA-sequencing experiments were performed on two RNA pools from two independent cultures of WT and P2Y2 KO cADSC after 7 days in proliferation medium and daily stimulated or not with 100 µM UTP. Comparison of RNA-sequencing data identified 977 genes regulated by UTP only in WT ADSC and not in P2Y2 KO cADSC. GO detailed analysis revealed various enriched biological processes related to the 977 UTP target genes. (B) Confirmation of specific UTP target genes by qPCR experiments. Ccl5, Cxcl5, and Saa3 mRNAs are upregulated, and Ccl12 mRNA is downregulated, in UTP-treated versus untreated cADSC in RNA-sequencing and qPCR experiments. Quantification of Ccl5, Cxcl5, Saa3, and Ccl12 mRNA level was performed by qPCR in at least six independent cADSC cultures and normalized to Rpl32 mRNA level. (C) UTP increases CCL5, CXCL5, and SAA3 release, and inhibits CCL12 release by cADSC. CCL5, CXCL5, SAA3, and CCL12 protein level was measured in the supernatants of cADSC cultures after 7 days in proliferation medium with or without daily stimulation with UTP 100 µM. ELISA data were obtained for six to nine independent cADSC cultures. Values represent mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001.